Infectious Disease Research Center

Bio-imagerie

Microscopy

The Bio-imaging platform is equipped with several microscopes with different applications. By clicking on each of the images, you will be able to view the details for each microscope.

*The photos of the microscopes were taken with an exposure time of 30 seconds which makes the illumination appear more intense.

For more information on the microscopes, their applications or to know the rates for the use of the different devices, please contact Julie-Christine Lévesque by email at jc.levesque@crchudequebec.ulaval.ca or by phone at 418 525-4444 ext. 48283.

Quorum Wave FX - Rotating Disc Confocal Microscope (QUORUM #1)

Quorum Wave FX - Rotating Disc Confocal Microscope (QUORUM #1)

Brand

Quorum Technologies

Model

Wave FX-Borealis – Leica DMI 6000B

Applications

Confocal microscopy,  Live cell imaging (Pathology Devices LiveCell3 environmental chamber), FRAP (Mosaic system)

Software

Volocity, Metamorph

Laser

Laser diodes : 440nm, 491nm, 561nm, 642nm

Détails

Objectifs

10X HC PL Apo NA 0.4 – 20X HC Plan Apo NA 0.70 Ph2 – 40X HCX PL Apo NA: 1.25-0.75 Oil – 63X HCX PL Apo NA 1.3 GLYCEROL 37°C

Filtres
Epi-fluorescence

DAPI – CFP – GPF – RFP

Mode Confocal

Filter wheel 1: 536/40 – 593/40 – 624/40 – 692 – Triple 491/561/635
Filter wheel 2: 470/30 – 520/35 – 542/27 – 593/40 – dual 491/561

Caméra

Image EM (Hamamatsu, 512×512 pixels) et Orca-R2 (Hamamatsu, 1344×1024 pixels), Caméra couleur RTV de Qimaging

Nikon Fluorescence Microscope

Nikon Fluorescence Microscope

Brand

Nikon

Model

Eclipse TE300

Applications

Epi-fluorescence, phase contrast (up to 40X)

Software

Image-Pro Plus 5.0

 

Détails

Objectifs

Plan Fluor 4X /0.13 PhL (contraste de phase) – Plan Fluor 10X/0.30 Ph1 (contraste de phase) – Plan Fluor 20X/0.45 Ph1 (contraste de phase)  – Fluor 60X/0.85 (sur demande) – Plan APO VC 100X H/1.40 Oil

Filtres

– Filtre UV (DAPI, BFP, Alexa 350) Excitation : 340-380 nm, Émission : 435-485 nm
– Filtre Bleu (GFP, Alexa 488, FITC, YFP) Excitation : 460-500 nm, Émission : 510-560 nm
– Filtre Vert (Alexa 555, Alexa 568, TexasRed, Cy3)) Excitation : 532-588 nm, Émission : 607-683 nm

Caméra

Coolsnap Fx (Roper Scientific)

Nikon stereo microscope with color camera

Nikon stereo microscope with color camera

Brand

Nikon

Model

SMZ800

Applications

10X to 63X sample observation, optic fibre illumination. Comes with a base attachment for illumination directly below the sample.

 

Détails

Caméra

DS-Fi1 avec système d’acquisition DS-L2. La caméra peut être déplacée d’un microscope à l’autre (autorisation requise).

Capture

Enregistrement des images directement sur une clé USB ou carte mémoire

Quorum Wave FX - Rotating Disc Confocal Microscope (QUORUM #2)

Quorum Wave FX - Rotating Disc Confocal Microscope (QUORUM #2)

Brand

Quorum Technologies

Model

Wave FX-Borealis – Olympus BX 81

Applications

Confocal microscopy, live cell imaging (Live Cell Instrument Environmental Chamber), laser microdissection (MMI CellCut System) and capillary cell sorting

Software

MetaMorph, Volocity

Laser

Laser diodes : 405nm, 491nm, 561nm, 642nm

 

Détails

Objectifs

10X UPlanS NA 0.40 – 20X UPlanSApo NA 0.75 – 40X UPlanSApo NA 0.95 – 60X Oil PlanApo N NA 1.42

Filtres
Épi-fluorescence

DAPI – GFP – RFP

Mode Confocal

Filter wheel 1 : 536/40 – 593/40 – 624/40 – 692 – Triple 491/561/635

Filter wheel 2 : 470/30 – 520/35 – 542/27 – 593/40 – dual 491/561

Filter wheel 3 (live) : 520/25 – 540/30 – 600/37 – 605/70 – 692/40 – 780-62

Caméra

Image EM (Hamamatsu, 512×512 pixels), Orca-R2 (Hamamatsu, 1344×1024 pixels)

FEI Tecnai Spirit G2 electronic microscope

FEI Tecnai Spirit G2 electronic microscope

Brand

FEI

Model

TECNAI Spirit G2 Biotwin

Applications

This transmission electron micropscope allows high-resolution visualisation of biological samples. It can also be used for 3D electron tomography and cryo-microscopy.

Software

Microscope user interface, AMT v601

Détails

Voltage

80-120 kV

Caméra

AMT (Orca HR, 11 Megapixels), bottom mount