The Bio-imaging platform is equipped with several microscopes with different applications. By clicking on each of the images, you will be able to view the details for each microscope.
*The photos of the microscopes were taken with an exposure time of 30 seconds which makes the illumination appear more intense.
For more information on the microscopes, their applications or to know the rates for the use of the different devices, please contact Julie-Christine Lévesque by email at jc.levesque@crchudequebec.ulaval.ca or by phone at 418 525-4444 ext. 48283.
Quorum Technologies
Wave FX-Borealis – Leica DMI 6000B
Confocal microscopy, Live cell imaging (Pathology Devices LiveCell3 environmental chamber), FRAP (Mosaic system)
Volocity, Metamorph
Laser diodes : 440nm, 491nm, 561nm, 642nm
10X HC PL Apo NA 0.4 – 20X HC Plan Apo NA 0.70 Ph2 – 40X HCX PL Apo NA: 1.25-0.75 Oil – 63X HCX PL Apo NA 1.3 GLYCEROL 37°C
DAPI – CFP – GPF – RFP
Filter wheel 1: 536/40 – 593/40 – 624/40 – 692 – Triple 491/561/635
Filter wheel 2: 470/30 – 520/35 – 542/27 – 593/40 – dual 491/561
Image EM (Hamamatsu, 512×512 pixels) et Orca-R2 (Hamamatsu, 1344×1024 pixels), Caméra couleur RTV de Qimaging
Nikon
Eclipse TE300
Epi-fluorescence, phase contrast (up to 40X)
Image-Pro Plus 5.0
Plan Fluor 4X /0.13 PhL (contraste de phase) – Plan Fluor 10X/0.30 Ph1 (contraste de phase) – Plan Fluor 20X/0.45 Ph1 (contraste de phase) – Fluor 60X/0.85 (sur demande) – Plan APO VC 100X H/1.40 Oil
– Filtre UV (DAPI, BFP, Alexa 350) Excitation : 340-380 nm, Émission : 435-485 nm
– Filtre Bleu (GFP, Alexa 488, FITC, YFP) Excitation : 460-500 nm, Émission : 510-560 nm
– Filtre Vert (Alexa 555, Alexa 568, TexasRed, Cy3)) Excitation : 532-588 nm, Émission : 607-683 nm
Coolsnap Fx (Roper Scientific)
Nikon
SMZ800
10X to 63X sample observation, optic fibre illumination. Comes with a base attachment for illumination directly below the sample.
DS-Fi1 avec système d’acquisition DS-L2. La caméra peut être déplacée d’un microscope à l’autre (autorisation requise).
Enregistrement des images directement sur une clé USB ou carte mémoire
Quorum Technologies
Wave FX-Borealis – Olympus BX 81
Confocal microscopy, live cell imaging (Live Cell Instrument Environmental Chamber), laser microdissection (MMI CellCut System) and capillary cell sorting
MetaMorph, Volocity
Laser diodes : 405nm, 491nm, 561nm, 642nm
10X UPlanS NA 0.40 – 20X UPlanSApo NA 0.75 – 40X UPlanSApo NA 0.95 – 60X Oil PlanApo N NA 1.42
DAPI – GFP – RFP
Filter wheel 1 : 536/40 – 593/40 – 624/40 – 692 – Triple 491/561/635
Filter wheel 2 : 470/30 – 520/35 – 542/27 – 593/40 – dual 491/561
Filter wheel 3 (live) : 520/25 – 540/30 – 600/37 – 605/70 – 692/40 – 780-62
Image EM (Hamamatsu, 512×512 pixels), Orca-R2 (Hamamatsu, 1344×1024 pixels)
FEI
TECNAI Spirit G2 Biotwin
This transmission electron micropscope allows high-resolution visualisation of biological samples. It can also be used for 3D electron tomography and cryo-microscopy.
Microscope user interface, AMT v601
80-120 kV
AMT (Orca HR, 11 Megapixels), bottom mount